Quantitative Point of Care testing developing is significant especially in resource and time-limited settings. 
Affordable technologies that can enable locally to improve healthcare, environmental safety, animal health, and food. Diagnosis is the first step to treat a condition: yet, the major stumbling block in monitoring and controlling diseases/contaminations remains delivering simple, low-cost and robust diagnostic tests.

Dipstick and lateral-flow formats have dominated rapid diagnostics over the last three decades. These formats gained popularity in the consumer markets due to their compactness, portability and facile interpretation without external instrumentation. 

However, lack of quantitation in measurements has challenged the demand of existing assay formats in consumer markets. Recently, paper-based microfluidics has emerged as a multiplexable point-of-care platform which might transcend the capabilities of existing assays in resource-limited settings. However, paper-based microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental
monitoring and food safety. Currently, in its early development stages, paper-based microfluidics is considered a low-cost, lightweight, and disposable technology. (1)

Fast -Diag is developing new approach to create semiquantitative or quantitative PoС affine chromatography test. 


First commercial approach - Early Bovine Pregnancy testing

The problem of early pregnancy testing costs billions of dollars. In current conditions the breeding is mainly artificial but still does not allow to reach rate after fertilization more than 60-65%. (2) Earlier you detect non-pregnant cows, so earlier youl do one more breeding to achieve higher rate. Dairy and meat farms incur large financial costs in the preparatory period and when feeding pregnant animals in the period before fertilization is confirmed.  The methods today widely used to detect bovine non-pregnancy are well described in movie below.

Project Aim: Development of an easy-to-use cheap rapid cattle pregnancy test to increase the efficiency of its reproduction due to early detection of the bovine non-pregnancy.

The most adequate method of non pregnancy detection - is progesterone level detection. (6It gives the results оn 21 day and gives very precise results in non-pregnancy detection. But currently the cost to perform milk/blood test varies up to $5 per test and test needs to be performed by professionals.(4)(5)  

Our Solution: There is a demand on cheap (<$1,5) PoC milk express method for determining the cow's pregnancy on 21 day after the breeding which will reduce the cost during a false pregnancy. The existing techniques and design to develop PoC chromatography devices could not reach need prices (11) which are higher $6.  

We are developing a cheap paper chromatography device which will allow to non - professional farmer himself monitor the progesterone milk level and to invite veterinary specialist to perform blood tests in heifers only. The next stage we shall develop the paper chromatography blood test also for heifers. 

Competing: There were several attempts to create PoC devices for on farm progesterone testing (7, 8, 9) based on lateral flow design(10). There are the attempts to create human like pregnancy test based on luteinizing hormone level (3, 12) and it still costs higher $3 per analysis . However urine testing is technically very difficult and you can look movie below to get a fun with such procedure.